She told investigators her legs felt like water, like Jello. She woke up inside a garage, with a group of kids who were already on police radar.
"These kids we've had an eye on for awhile -- involved in many types of activity.
The progeny of the transplanted cells could be serially transplanted into the appropriate recipients multiple times; however, unlike preneoplastic cells or neoplastic cells, the normal cells always senesced after multiple serial transplants, generally five to eight transplant generations .
This was interpreted as indicating that the proliferative activity was a finite property of the stem cells.
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Nevertheless, re-examination of the literature on the transplantation biology of mammary gland regeneration reveals that a complex, combinatorial interaction between variously differentiated mammary epithelial cells and the mammary fat pad stroma is indispensable to this process.
In the present article, these issues are reviewed and discussed to provide a greater understanding of the complexity of these multiplex interactions.
The cleared fat pad transplantation technique allowed the transplantation and growth of normal mammary cells into their normal anatomical site and under the influence of a normal physiological environment.
Using this method, they demonstrated that the normal mammary gland contains cells that will grow and fill the fat pad with a normal ductal mammary tree and will respond to hormones with a normal differentiation program .